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Hypo-glycolysis metabolism in ECs may also contribute to endothelial dysfunction in atherosclerosis

HEK293 cells transfected with HA-tagged WT ghrelin receptor or mutants were seeded at a density of 4 10 4 cells per well into 96-well culture plates and incubated for 24 h at 37 C in 5% CO 2 .The cells were then incubated with 2 mol/L Fluo-4 AM in HBSS (5.4 mmol/L KCl, 0.3 mmol/L Na 2 HPO 4 , 0.4 mmol/L KH 2 PO 4 , 4.2 mmol/L NaHCO 3 , 1.3 mmol/L CaCl 2 , 0.5 mmol/L MgCl 2 , 0.6 mmol/L MgSO 4 , 137 mmol/L NaCl, 5.6 mmol/L D-glucose and 250 mol/L sulfinpyrazone, pH 7.4) at 37 C for 40 min

A single treatment can offer a temporary boost, but sustained benefits require repeated dosing that keeps plasma concentrations elevated over time

Rogol AD, Swerdloff RS, Reiter EO, Ross JL, ZumBrunnen TL, Pratt GA, Brennan JJ, Benesh J, Kan-Dobrosky N, Miller MG

Its also formulated with lidocaine, a local anesthetic to help make the procedure as painless as possible

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