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This work was funded by the Clinical Research Special Project of Shandong University of Traditional Chinese Medicine (LCKY202429), the Basic Research Projects of Yantai Science and Technology Innovation Development Plan (2023JCYJ062), and the High-level Talent Research Start-up Fund Project of Shandong Xiehe University (SDXHQD2024054)

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Evaluation of cell apoptosis by Annexin V assay To investigate the possible role of MVs treatment on apoptosis induction in fibroblasts, on days 1, 3, and 5 of culture, Annexin V-FITC and PI (EXBIO, Vestec, Czech Republic) were utilized for double-staining of cells, followed by flow cytometry (BD Biosciences, San Jose, CA, USA) analysis

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