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The actual voltage, matching the car battery's voltage, will be approximately 12.5 volts when dormant (less in cold conditions), approximately 14.5 volts when the engine and the alternator/generator are operating (more when cold), and may briefly drop as low as 56 volts during engine start due to the high temporary battery current usage

Instead, saliva was used as a surrogate matrix, which is widely accepted as reflecting the overall oral microbial community, as it contains microorganisms shed from multiple intraoral niches, including both supragingival and subgingival sites, as we and others have previously demonstrated44

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