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For RNA-seq analysis of bulk kidney and gastrocnemius skeletal muscle samples from WT and Klotho KO male mice, 6 biological replicates per group were utilized for every tissue, resulting in the total of 24 samples

Besides the oxidative stress response, PARP1 plays crucial roles in key cellular and physiological processes such as chromatin remodeling, inflammation, DNA repair, regulation of transcription, as well as induction of cell death (reviewed in [117])

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and in neuronal cultures, it supports BDNF signaling and mitochondrial function