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Cells were harvested in 200 L PBS + 0.1% BSA and subjected to flow cytometry analysis to examine oxidation of BODIPY 581/591 C11, a reporter for lipid ROS levels in cells

D.ShiotaM.QianJ.HarrisB

Govindarajan VS, Sathyanarayana MN

For the NAC rescue experiment, mice received intraperitoneal administration of 200 mg/kg of body weight NAC (A105422, Aladdin) in phosphate-buffered saline (pH 7.4) every second day for two weeks

Beyond promoting detoxification via bile excretion, TUDCA offers cell-protective effects: it stabilizes mitochondrial membranes, reduces oxidative stress and endoplasmic-reticulum (ER) stress, and blocks apoptosis (cell death) in hepatocytes

Side Effects of Glutathione The side effects often depend on the method of administration: Oral Supplements: Generally, oral glutathione has fewer side effects, but some individuals may experience skin rashes or mild stomach pain