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Ct values obtained from RT-qPCR results performed with primers with primer activities between 1.9 and 2.2 were normalized using the 2-Ct method according to Livak and Schmittengen 73 criteria with the Ct value obtained for the specified reference gene (housekeeping)

doi: 10.1073/pnas.011513098 204 OConnorTGWinterMAHunnJCarnahanJPressmanEKGloverVet al

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(RPB) Core grant (to Duke Eye Center)

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doi: 10.1016/j.molcel.2010.01.017