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1) based upon medical history and clinical condition, where the microbiological workup subsequently confirms that bacterial infection is unlikely, PCT levels that are initially low or decreasing over time can help confirm the absence of a bacterial infection and allow for ongoing treatment without antibiotics or safe discontinuation of antibiotics if started

After treatment, cells were washed in phosphate buffered saline (PBS) containing 1% bovine serum albumin (BSA), detached from the plate using Accutase and transferred to FACS tubes

So, thats where you should be prepared to feel some discomfort

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The Lys17 side chain is a C20 fatty diacid attached through a PEG2 spacer and a -glutamic acid linker