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In flow cytometry, cells were collected and resuspended in 50ul FACS buffer (PBS + 2.5% FBS + 0.5% sodium azide) and TruStain FcX was used TM Human monocyte enrichment cocktail (Stemcell Technologies, Vancouver, Canada) monocytes were isolated from whole blood of healthy donors according to the manufacturer's instructions

Bull Mar Sci 53:10661077 Savin-Amador M, Hernandez-Rubio JS, Caballero-Cosio MA et al (2024) Evaluation of the antibacterial activity of the skin mucus of the common octopus Octopus bimaculatus against pathogenic bacteria of farm animals