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Once symptoms clear, reintroduce 5-amino-1MQ at half the original dose (e.g., 25mg instead of 50mg daily) and titrate upward slowly over 23 weeks to assess tolerance

Circulation 111, 106112

For the wash-out experiments cells were similarly handled but washed three times with Opti-MEM + 1% FBS (100 l) before adding 100 l fresh media with 6 ng/ml PMA solution without any inhibitor for 6 h (Wash-out)

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Future investigations on how the biological clock governs cell proliferation and growth and on the potential implications for the onset of human diseases are warranted