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PMID: 40089135 Genes (Basel)The VEGFA-Induced MAPK-AKT/PTEN/TGF Signal Pathway Enhances Progression and MDR in Gastric Cancer

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For Western blot analysis, 30 g protein per sample were mixed with loading buffer, denatured at 95 C for 5 min (PDHA1 and pPDHA1) or at 37 C for 20 min (OXPHOS complexes) and separated on a TGX Stain-Free Precast gradient gel (Bio-Rad Laboratories GmbH, Germany)

mTOR, Rictor, Raptor, DEPTOR, PRAS40, and FKBP12 were probed

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Liposomes consist of one or more phospholipid bilayers that can encapsulate both hydrophilic and hydrophobic drugs